TLC-densitometric method for quantitation of Lurasidone hydrochloride in nanoemulsion, microemulsion, for equilibrium solubility and ex vivo diffusion studies.
Abstract
A new TLC-densitometric method has been successfully developed for quantitation of Lurasidone hydrochloride (LURHA) in pharmaceutical formulations. LURHA was well separated from sample matrix and degradation product using TLC plate; precoated with silica gel G 60F254 on aluminium sheet as a stationary phase and mixture of hexane: ethyl acetate (6:4 v/v) as a mobile phase. LURHA was quantified by densitometric analysis at 323 nm. The method was found to give compact bands for the drug (Rf = 0.47 ± 0.01). Statistical analysis of the data showed that the method is specific, precise, accurate, reproducible, and selective for the analysis of LURHA. The TLC-densitometric method was successfully applied for the determination of equilibrium solubility of LURHA in various excipents and ex vivo diffusion study through sheep nasal mucosa from in-house developed formulations (solution, nanoemulsion, mucoadhesive nanoemulsion and microemulsion).
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L. Citrome, J. Cucchiaro, K. Sarma, R. Silva, S. Tsuchiya, A. Loebel. Int. Clin. Psychopharmacol. 2012, 27, 165–176.
A. Loebel, J. Cucchiaro, K. Sarma, L. Xu, C. Hsu, A.H. Kalali, A. Pikalov, S.G. Potkin. Schizophrenia Res. 2013, 145, 101–119.
S. S. Muvvala, V. N. Ratnakaram. Res. J. Pharm. Bio. Chem. Sci. 2013, 4, 609–617.
N. K. Joshi, N. Shah, M. Dumasiya, A. Patel. Int. J. Pharm. Sci. 2012, 3, 2643–2653 (2012).
P. Ravisankar, G. Rajyalakshmi, D.C. Dasu, P. Srinivasa, P.V. Reddy. Der Pharm. Sinica. 2014, 5, 1–7.
N. Mali, J. Patel, M. Patel. Int. J. Res. Pharm. Sci. 2012, 2, 44–50.
S. A. Patel, K. Y. Patel. Am. J. Pharm. Res. 2014, 4, 602–610.
P.J. Chhabda, M. Balaji, V. Srinivasarao, K.M. Rao. Int. J. Pharm. Res. Dev. 2013, 5: 103–114.
A. R. Polawar, M. C. Damle. Int. J. Res. Pharm. Chem. 2014, 4, 327–332.
K. Y. Patel, S. A. Patel. Int. J. Pharm. Drug Anal. 2014, 2, 169–173.
D. Katasani, B. Srinu, R. Bala. Drug Inven. Today. 2011, 3, 305–308.
P. Ravisankar, G. Rajyalakshmi, D.C. Dasu, P. Srinivasa, P.V. Reddy. World J. Pharm. Res. 2014, 3, 453–466.
C. Yoon-Jee, K. Tae-Sung, L. Kyeong-Ryoon. Chromatographia. 2012, 75, 1117–1128.
K. Tae‐Sung, K. Soo‐Jin, L. Jongjoo, H. Dong‐Jin, B. Myoungki, M. Hongsik. Biomed. Chromatogr. 2011, 25, 1389–1394.
A. R. Polawar, M. C. Damle. Pharm. Sci. Mon. 2014, 5, 131–144.
M. Gopani, R. B. Patel, M. R. Patel, A.B Solanki. J. Liq. Chromatogr. Rel. Tech. 2014, 37: 2420–2432.
R. B. Patel, M. R. Patel, N. Dubey, N. Dubey, B. Patel. J. Food Drug Anal. 2012, 20, 561–570.
R. B. Patel, M. R. Patel, K. K. Bhatt, B. Patel. Anal. Methods. 2010, 2, 525–531.
D.H. Shewiyo, E. Kaale, P.G. Risha, B. Dejaegher, J. Smeyers-Verbeke, Y. Vander Heyden. J. Pharm. Biomed. Anal. 2012, 66, 11–23.
R. B. Patel, M. R. Patel, B. G. Patel, J. AOAC Int. 2010, 93, 811–819.
R. B. Patel; M. R. Patel; B. G. Patel, In High performance thin layer chromatography (HPTLC), M. M. Srivastava Ed; Springer-Verlag Berlin Heidelberg: New York, 2011, Chapter 3, pp. 41–54.
R. B. Patel, M. R. Patel, T. S. Goswami, Thai J. Pharm. Sci. 2014, 38, 168–175.
International Conference on Harmonization (ICH). Validation of Analytical Procedure: Text and Methodology Q2 (R1), IFPMA: Geneva, Switzerland, 2005.
R. B. Patel, M. R. Patel, K. K. Bhatt, B. G. Patel. Drug Del. 2016, 23, 346–354.
R. B. Patel, M. R. Patel, K. K. Bhatt, B. G. Patel. J. Drug Del. Sci. Tech. 2013, 23, 561–567.
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