Determination of degradation of barakol in extracts of Senna siamea and its herbal recipes

Abstract

Keywords: Degradation, barakol, Senna siamea

Objectives: The reverse phase high performance liquid chromatography was used to analyze the degradation of barakol in extracts of leaf and flower of Senna siamea as well as in the S. siamea herbal recipe.

Methods: Mature leaf and young flower of S. siamea were harvested from different five provinces. Each sample was extracted in 95% ethanol. S. siamea was mixed with S. tora, Leonurus sibiricus, or both of them and extracted in each boiling water, water at ambient temperature, 60% ethanol, or 95% ethanol by ultrasonication. Barakol content and degradation in fresh extracts preparation and storage in desiccator at ambient temperature with light protecting for 10 months were analyzes and compared.

Results: After 10 months storage, degradation of barakol in S. siamea leaf harvested from different places was ranged from 50.29-90.11% (average value approximately 64%). While, S. siamea flower, degradation of barakol was ranged from 23.29-67.84% (average value approximately 49%). When used ethanol as extraction solvent, the barakol induced the high degradation in  Recipe 1 (1 part of leaf of S. siamea, 1 part of seed of S. tora, and 2 parts of aerial part of Leonurus sibiricus) and Recipe 2 (1 part of leaf of S. siamea and 3 parts of seed of S. tora), degraded more than 90%. But, 95% ethanol could not extracted barakol from Recipe 3 (1 part of leaf of S. siamea and 3 parts of aerial part of L. sibiricus). 

Conclusion: Barakol in leaf extracts highly degraded more than in flower extracts. Furthermore, when used ethanol as extraction solvent for herbal recipes, the barakol was high degraded compared to boiling water. In summary, plant harvested places, part used, composition of herbal recipe, and extraction solvent affected degradation of barakol in extracts.