Anti-oxidant activities and polyphenolic compounds of Longan (Dimocarpus longan Lour) peel and seed extracts
Abstract
Keywords: Longan (Dimocarpus longan Lour), anti-oxidant activity, polyphenolic compounds
Objectives: The objectives of the present study were to investigate the antioxidant activity and determine major polyphenolic compounds from Longan (Dimocarpus longan Lour) peel and seed extracts.
Methods: Longan peels and seeds were extracted with 20% and 95% ethanol. The combined filtrates of ethanol solution were evaporated under reduced pressure at room temperature. Anti-oxidative activity was measured using the 2,2-Diphenyl-1-picrylhydrazin (DPPH) radical scavenging assay compared with ascorbic acid. Phytochemical screening was investigated using reversed-phase TLC technique. Major components of polyphenolic compounds were determined by HPLC technique.
Results: The IC50 of antioxidant activity of 20% and 95% ethanol crude extracts of longan peels and seeds were in the range of 0.68-1.73 g/mL nearly to ascorbic acid (1.37 g/mL). Reversed- phase TLC profile of the crude extracts showed main spots equivalent to the authentic standards of polyphenolic compounds such as gallic acid, ellagic acid and flavonoids compound such as quercitin. The amounts of gallic acid, corilagin and ellagic acid ranged from 1.20 to 23.86 mg/g of the dried crude extracts.
Conclusion: From this study, the crude ethanolic extracts of longan peels and seeds exhibit good antioxidant activity. The obtained extracts were consisted of polyphenolic compounds, namely gallic acid, corilagin and ellagic acid. Further investigations on insomnia and immunomodulation are needed to confirm the biological activities of the extracts that can be applied to food supplement purpose.
Objectives: The objectives of the present study were to investigate the antioxidant activity and determine major polyphenolic compounds from Longan (Dimocarpus longan Lour) peel and seed extracts.
Methods: Longan peels and seeds were extracted with 20% and 95% ethanol. The combined filtrates of ethanol solution were evaporated under reduced pressure at room temperature. Anti-oxidative activity was measured using the 2,2-Diphenyl-1-picrylhydrazin (DPPH) radical scavenging assay compared with ascorbic acid. Phytochemical screening was investigated using reversed-phase TLC technique. Major components of polyphenolic compounds were determined by HPLC technique.
Results: The IC50 of antioxidant activity of 20% and 95% ethanol crude extracts of longan peels and seeds were in the range of 0.68-1.73 g/mL nearly to ascorbic acid (1.37 g/mL). Reversed- phase TLC profile of the crude extracts showed main spots equivalent to the authentic standards of polyphenolic compounds such as gallic acid, ellagic acid and flavonoids compound such as quercitin. The amounts of gallic acid, corilagin and ellagic acid ranged from 1.20 to 23.86 mg/g of the dried crude extracts.
Conclusion: From this study, the crude ethanolic extracts of longan peels and seeds exhibit good antioxidant activity. The obtained extracts were consisted of polyphenolic compounds, namely gallic acid, corilagin and ellagic acid. Further investigations on insomnia and immunomodulation are needed to confirm the biological activities of the extracts that can be applied to food supplement purpose.
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