In vitro antioxidant activity of electrospun polyvinyl alcohol nanofiber mats containing stingless bees’ propolis extracts

Abstract

Keywords: Stingless bees’ propolis, Polyvinyl alcohol, Nanofiber, Antioxidant

Objectives: The goal of this study was to develop electrospun nanofiber mats containing stingless bee’s propolis extracts and evaluated in vitro antioxidant activity via (2,2-diphenyl-1-picryhydrazyl (DPPH)) method.

Methods: Polyvinyl alcohol (PVA) was selected as polymer. The stingless bee’s propolis extracts with the concentration of 0.5%, 1% and 2% wt alpha-mangostin were incorporated to PVA solution. Mixture solution was prepared to nanofibers via electrospinning process. The morphology and diameter of the electrospun nanofiber were observed using scanning electron microscope (SEM). The amount of alpha-mangostin was determined by high performance liquid chromatography (HPLC).The anti-oxidative activity of (2,2-diphenyl-1-picryhydrazyl (DPPH)) method were evaluated.

Results: The results showed that the diameters of the fibers were 184.0 ± 28 - 226.3 ± 64 nm and no crystal of the extract was detected in all concentrations. The α-mangostin content in electrospun nanofibers prepared by incorporating 0.5%, 1% and 2% wt were 64.2 ± 5.8 % 56.2 ± 5.3 % and 48.8 ± 1.9 % respectively. The antioxidant activities (IC₅₀) which evaluated by DPPH method of all electrospun nanofibers were 1.12-1.87 mg/ml.

Conclusion: In summary, Electrospun PVA nanofibers containing stingless bee’s propolis may have potential for pharmaceutical application.