Phytofabrication of silver nanoparticles using Horse Gram (Dolichos biflorus L.) seed extract and assessment of its bactericidal and antioxidant activities

Abstract

The “Phytofabrication” of silver nanoparticles receives bigger attention due to their

fascinating properties. This study deals with the green mediated synthesis of silver nanoparticles

using horse gram (Dolichos biflorus L.) seeds extract and assessment of its bactericidal activities.

The green synthesized silver nanoparticles were characterized using UV–Vis absorption

spectroscopic analysis, FTIR, EDX, XRD and SEM. In UV- vis spectroscopic analysis, the

observation of strong peak at 442 nm. The XRD pattern showed crystalline in nature of silver

nanoparticles. The average estimated particle size of the sample was 29.09 nm. Scanning

Electron Microscopy (SEM) revealed the cubic in nature of silver nanoparticles. FTIR analysis

showed the possible functional groups involved in the AgNPs formation. The EDAX result

showed a large peak of silver that confirmed its presence in the suspension. Further silver

nanoparticles were evaluated for their antibacterial activity against different human pathogens by

standard well diffusion method. The highest antibacterial activity was observed against B.

subtilis followed by P. aeroginosa, Klebsiella sp., E. coli, Staphylococcus sp., and no activity

was noticed against Proteus sp. The percentage of inhibition of free radicals increased with

increase in concentration of substrates. The concentration 60 µl of nanoparticles showed 99% of

scavenging activity. The present study suggests that horse gram mediated silver nanoparticle is

easy and eco-safe with potential bactericidal and antioxidant activities.