Relationship between the structure and immunogenicity of ceftriaxone

Abstract

Ceftriaxone is one of the most reported cause allergic reaction due to frequently prescribed.⁽¹⁾ Once the patients are suspected to ceftriaxone allergy, we must be concerned with cross-allergic reaction to cephalosporins and other β-lactam antibiotics. The relationship between structure and immunogenicity of β-lactam antibiotics could be beneficial for drug treatment selection. Several studies have attempted to reveal the structure of cephalosporins that involved the allergic reaction and cross reaction to other β-lactam antibiotics by skin test, re-challenge test, and radioimmunoassay on IgE determination.^(2-6) These methods have disadvantages such as invasion or limitation of time interval from allergen exposure.⁽⁷⁾ The in vitro immunoassay for instance enzyme-linked immunosorbent spot (ELISPOT) is an optional method to investigate the allergic reaction due to specificity and non-invasive.⁽⁸⁾ Our objective is to investigate the relationship between structure of ceftriaxone and its immunogenicity using ELISPOT IFN-γ assay. The tested compounds are including native molecule of ceftriaxone, its side chain moieties, degradation products, and other native cephalosporins.