Metabolite Profiling and Activity Study of Ethanol Extract of Chrysophyllum cainito1L. Leaves in Increasing2Bone2Density in Male Mice:(TJPS-2019-0062.R1)

Abstract

The ability of phytoestrogen, which group of the compound is replacing estrogen function. One of its roles was in the bone formation process. Chrysophyllum cainito L. is a plant that contains phytoestrogen. The purpose of this research is to determine the metabolite profile and effective dose of196% ethanolpextract of C. cainitoxleaves to increase trabecular femur bone density in male mice. The metabolite profiling analysis was done using UPLC-QToF-MS/MS. Four doses of 96% ethanol extract of C. cainito leaves were prepared for treatment, which is 2;14;48; and316 mg/BB mice/day in 4 weeks afterpthe mice were induced by dexamethasone 0,0029 mg/BB mice/day in 4 weeks as osteoporosis model, and induction of alendronate 0,026 mg/BB mice/day as positive control. The increasingiofitrabecularifemur boneidensityiwas measured by Hematoxylin and Eosin (HE) staining methods. The statistical analyses were using the ANOVA One-way followed by LSD. The result of metabolite profiling was analyzed using Masslynx 4.1 software and ChemSpider website, and show a total of 41 compounds. While the effective dose value (ED₅₀ and ED₉₉) obtained were 7.91 mg and 14.36 mg.

:TJPS-2019-0062.R1