Simple and Rapid Spectrophotometric Method for Quality Determination of Roselle (Hibiscus sabdariffa)

Tasamaporn Sukwattanasinit, Jankana Burana-osot, Uthai Sotanaphun

Abstract


A simple and rapid spectrophotometric method has been developed to determine the quality of roselle (Hibiscus sabdariffa).  The absorbance at 530 nm (coloring intensity) and the ratio between the absorbances at 380 and 530 nm (degradation index) of its 50% methanol extract were suggested as the criterion parameters to discriminate between the qualified and unqualified samples.  The contents of total anthocyanins and total phenolic compounds were also estimated by the partial least square (PLS) and multiple linear regression (MLR) models established from the absorbances at 330, 380 and 530 nm.  Their reliability was indicated by the low root-mean-square error of prediction (RMSEP = 0.11-0.13) and high correlation between the actual and prediction values of the tested samples (R = 0.99).


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References


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