The Study of Physical Properties and Formulation of Self-assembling Nanogel from Grafted Hydrophilic Polymer Backbone as Stabilization System for Curcumin

Abstract

The aim of this work is to formulate and study the thermoresponsive system with hyaluronic acid (HA) as a polysaccharide backbone grafted with poly-(N-isopropylacrylamide) (pNIPAM) for stabilization of highly sensitive molecule. The nanogel forming polymer was synthesized through EDC/NHS coupling reaction. The hyaluronic acid grafted pNIPAM (HA-g-pNIPAM) can be self-assembled to form gel particle. The synthesized HA-g-pNIPAM polymers were characterized by 1H NMR. Size, influence of media to forming nanogel, thermoresponsive behavior and influence of HA-g-pNIPAM nanogel network on curcumin loading capacity and stability were investigated by dynamic light scattering (DLS) and UV-VIS spectrophotometry. The results showed that the nanogel prepared in ultrapure water is appropriate in order to form nanogel with submicron-size range. The lower critical solution temperature (LCST) of HA-g-pNIPAM nanogels appeared at 33 ᵒC. This study also indicated the importance of polymer network as solubility and stability enhancer of curcumin which could be a model for other labile molecules or insoluble drugs.

:TJPS-2020-0041.R1