Acute and sub-acute toxicity profile of methanol leaf extract of Geophila obvallata on renal and hepatic indices in Wistar rats:(TJPS-2020-0071.R2)

Abstract

Introduction: Geophila obvallata (Rubiaceae) is a medicinal herb used for the treatment of diseases associated with oxidative damage. Objective: The study investigated the toxic effects of acute and sub-acute oral doses of methanol extracts of Geophila obvallata (GOE) in rats. Materials and Methods: In acute toxicity study, extract doses of 1600, 2900 and 5000mg/kg were orally administered. The rats were observed for signs of toxicity for two weeks. In sub-acute toxicity study, the extract was administered to rats at doses of 100, 500 and 1000mg/kg for 28 days, while control rats received only water. At the end of 28 days, rats were sacrificed. Organ weights, biochemical and histological parameters were determined using standard procedures. Results: In acute toxicity studies, the extract did not induce death after single dose administration. Hence, the LD50 was above 5000mg/kg. The results of sub-acute toxicity study showed no significant changes in body weights, organ weights, kidney function and histology. In contrast, there were significant changes in hematology and biochemical indices at higher extract doses of 500 and 1000mg/kg. Conclusion: These results suggest that GOE is non-toxic at a dose of 100mg/kg and can be used for therapeutic applications at an equivalent dose in humans.

:TJPS-2020-0071.R2