In vivo and In-silico Anti-arthritic studies of Chlorogenic acid from the rhizome of Lasia spinosa (L.) Thwaites (Araceae):(TJPS-2020-0235.R1)

Abstract

Aim: To examine the anti-arthritic function of MELS and chlorogenic acid in the rats of FCA mediated arthritis by Invivo and In silico studies. 
Methods: The MELS was subjected to acute oral toxicity in rats and tested against FCA induced arthritis in rats. The efficacy of MELS and CA against CFA-induced arthritis was subsequently tested for haematological, biochemical and In vivo anti-oxidant parameters in rats on the last day of the research. The IL-6 and TNF-α expressions in the paw tissue were determined by Western blotting technique. Ankle joint histopathological and radiological studies were also conducted. 
Results: MELS and CA dosage based on anti-arthritis, which was obvious in comparison with an arthritis control group with decreased paw volume, joint diameter and body weight. MELS and CA showed significant anti-arthritic activity by rising RBC and Hb levels and decreasing WBC and platelet levels. The anti-arthritis function has also been verified by the altered biochemical and anti-oxidant parameters. MELS (400 and 200 mg / kg) and CA (5, 10 mg / kg). 
Conclusion: This study confirms the ethnomedicinal use of Lasia spinosa rhizomes in the treatment of arthritis. It also indicates the strong anti-arthritic effects of CA.

:TJPS-2020-0235.R1