Application of proteoglycans from fish cartilage for the acceleration of burn wound healing
Abstract
Cartilage contains a variety of proteoglycans (PGs)that can accelerate wound healing. The aim of this study was to evaluate the efficacy of proteoglycans extracted from fish cartilage for the acceleration of burn wound healing. Second degree burn wounds were induced by placing an electrical hot plate set at 90°C for 10 s on the back of rats. Rats were randomly assigned to receive 1 g of cream base (control), 1% silver sulfadiazine (SSD), 1% PG, 2% PG, a combination of 1% SSD + 1% PG, or a combination of 1% SSD + 2% PG applied to burn wounds immediately after burning and once daily until day 27 post-burn. Wound healing was evaluated on days 3, 7, 14, 21 and 28. Histological analysis was performed on days 7, 14 and 21. The percentage wound healing after treatment with cream containing PG was significantly (p<0.001) higher than that in the control group on day 7 post-burn and thereafter. Histological analysis showed that the combination of 1% SSD + 2% PG had the highest efficacy in increasing re-epithelialization and neovascularization in burn wounds. We conclude that PG extracted from fish cartilage can accelerate and facilitate wound healing in rats. The combination of 1% SSD and 1% or 2% PG has high efficacy in accelerating and facilitating wound healing.
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