GC-MS fingerprints combined with chemometric analysis for the authentication of Morus alba leaves from Thailand:(TJPS-2021-0051.R1)

malai satiraphan, Aye Thida, Lawan Sratthaphut, Patcharawan Tanamartayarat, Onoomar Toyama

Abstract


 Introduction: Morus alba L. is one of the economic plants in Thailand. Several varieties are grown in various parts of the country. Different varieties from different regions may produce dissimilar chemical fingerprints. 

Objectives: The gas chromatography coupled to mass spectrometry (GC-MS) fingerprints of 2 varieties of M. alba leaves from 3 locations in Thailand, along with the tea products will be established. 

Materials and Methods: The GC-MS chromatograms combined with chemometric analysis were used to analyse the extracts of M. alba leaves and their tea products. 

Results: A total of 83 compounds comprising terpenes, saturated fatty acids, unsaturated fatty acids and benzofurans were found in M. alba leaves from both varieties. Fifteen compounds such as phytol, oleic acid and palmitic acid, with % peak area (detected at > 0.04%) from high to low, were found in var. Buriram 60. Eighteen compounds such as phytol, palmitic acid and oleic acid, respectively were found in var. Khun Pai. Six compounds, i.e. methyl palmitate, palmitic acid, methyl linolenate, phytol, oleic acid and stearic acid were found in the tea products. 

Conclusion: The GC-MS fingerprints were combined with chemometric analysis for the authentication and characterisation of 2 varieties of M. alba leaves from Thailand. 

Full Text:

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