Anti-Inflammatory Effect of Centella asiatica Extract on Prevented Aortic Intima-Media Thickening in Diabetic Rats
Abstract
Objectives: To explore the effect of C. asiatica extract on intima-media thickness, eNOS expression, and macrophage M1/M2 ratio in diabetic rats aorta. Materials and Methods: Wistar rats consisted of normal group and diabetic group which divided into negative control, positive control (captopril 50 mg/kg/day), C. asiatica extract (dose 250, 500, and 1000 mg/kg/day). The aorta was harvested after treatment for 8 weeks to measure intima-media thickness, eNOS mRNA expression, and macrophage M1/M2 ratio. Results: Dose 500 and 1000 mg/kg/day C. asiatica extract prevent aortic intima-media thickening (p < 0.05). Then dose of 1000 mg/kg/day prevent the increase of M1/M2 ratio (p < 0.05). Expression of eNOS mRNA did not give any significant results (p > 0.05). Conclusion: The extract of C. asiatica has anti-inflammatory effect and ability to prevent intima-media thickening on diabetic rats aorta.
Key words: Centella asiatica extract, Aortic Intima-Media Thickness, eNOS mRNA Expression, Macrophage Polarization, Diabetes Mellitus
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