A novel assay for evaluation of the total antioxidant capacity (TAC) using a nontoxic probe

Sirirat - Panich

Abstract


A novel and simple method for measuring total antioxidant capacity (TAC) was established. Herein, the new assay is based on generating the deep blue Imipramine (IMI) free radical by oxidising IMI with ammonium tetra vanadate (NH4VO3) in acid medium. Once the generated blue IMI radical is scavenged by the antioxidant compounds presenting in the samples, the blue colour of the oxidant disappears. Consequently, dropping in the absorption spectrum at 620 nm which can be detected by the spectrophotometric method. Also, sample throughputs and precision of the assay were enhanced by cooperating the novel analyse with the flow injection analysis (FIA) technique. The linearity of the assay was found to be up to 2,800 mg trolox equivalent L-1. The developed method was then applied to evaluate TAC in three different fractions of the G. Sootepensis’s extract. The proposed method was reliable, robust, inexpensive, environmentally friendly and suited for measuring TAC.

 


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