Antioxidant potential of wild Lagenaria siceraria (Molina) Standl.

Suraj B Patel, Usmangani A Attar, S G Ghane

Abstract


Abstract

Objective: The aim of the present study was to evaluate the phytochemicals and antioxidant activity of leaf and fruit of wild Bottle gourd.

Methods: Successive extraction of dried material was done by soxhlet apparatus and phytochemicals such as total phenolics, total flavonoids and total tannins were determined. The antioxidant assays were evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2’-Azino-bis-(3-ethyl) benzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity, metal chelating activity, ferric reducing antioxidant power (FRAP) and phosphomolybdenum assay.

Results: The present study revealed highest phenolics (195.15±11.64 TAE/g extract) and flavonoids (76.62±0.34 CE/g extract) in leaves extracted with ethanol. However, tannins were recorded higher in acetone leaf extract (225.98±10.56 CE/g extract). In fruit, maximum phenols (111.18±1.21 TAE/g extract) and tannins (47.14±2.23 CE/g extract) recorded in acetone and aqueous extracts respectively. FRAP activity was maximum in ethanol extract of leaf (395.79±2.00 mg Fe (II)/g extract) and fruit (196.00±3.18 mg Fe (II)/g extract). Among all the extracts, acetone extract of fruit displayed a strongest DPPH activity with IC50 values 711 ± 3.63 µg/mL.  ABTS radical scavenging was highest in ethanol (379.81±9.21 mg TE/g extract) and methanol (274.64±2.8 mg TE/g extract) extract of leaf and fruit respectively. On the other hand, aqueous extract exhibited highest metal chelating and phosphomolybdenum activity. Overall, ethanol and acetone were most suitable for the extraction of phytochemicals from the leaf. The antioxidant activities of leaf and fruit recorded significantly higher in the ethanol and methanol.

Conclusion: The work demonstrated that L. siceraria showed good antioxidant activity and may be the source of natural free radical scavenger.

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References


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