Hepatoprotective and choleretic activity of Tanacetum vulgare flowers extract

Oksana Mishchenko, Kateryna Kalko, Marharyta Zolotaikina, Tatyana Gontova, Victoria Mashtaler, Catherine Yurchenko, Lyudmyla Derymedvid, Anastasia Pozdniakova

Abstract


Objectives: The article presents the study of hepatoprotective and choleretic properties of Tanacetum vulgare flores dry extract (TVFDE) in vivo. Materials and Methods: The research was carried out using model of subchronic toxiс hepatitis in rats, caused by intragastric administration of 50% tetrahloromethane oil solution (0.4 ml per 100 g) and subcutaneous administration of 40% ethyl alcohol (1.3 ml per 100 g) within 4 days. An evaluation of the effect of TVFDE on the treatment-prophylactic regimen in doses of 25, 50, 75, 100 and 150 mg per kg was performed in 72 hours after the last administration of toxins. Results: It has been established that TVFDE has dose-dependent hepatoprotective and choleretic effects. TVFDE in doses of 75 and 100 mg per kg decreased thiobarbituric acid active products on 25% and 29% (p<0.05), increased the concentration of reduced glutathione on 40% and 34% (p<0.05), decreased the activity of alanine aminotransferase in 1.7 times (p<0.05) and 1.3 times (p<0.05) and the activity of aspartate aminotransferase in 1.3 (p<0.05 ) and 1.2 (p>0.05) times respectively versus control hepatitis. The use of TVFDE in doses of 50, 75, 100 and 150 mg per kg (excluding 25 mg per kg) contributed to the normalization of the bile volume and rate of bile secretion. Among all the studied doses, TVFDE only in a dose of 75 mg per kg showed the significantly improvement in bile composition: the bile acids content was significantly increased in 1.5 (p<0.05), cholesterol in 1.7 times (p<0.05) and influenced the total volume of allocated bile, which was significantly higher in 2.3 times (p<0.05) and contributed to alkaline phosphatase activity decrease in 1.4 times (p<0.05). Conclusion: TVFDE makes dose-dependent hepatoprotective and choleretic effects. Two most effective doses of TVFDE 75 and 100 mg / kg were determined. Based on the total assessment of the extract effect on the prooxidant-antioxidant imbalance, the activity of cytolysis and cholestasis, bile-synthetic and bile-secretory liver function TVFDE in a dose of 75 mg per kg was found to be most effective, therefore, it was selected for further research.

Key words: Tanacetum vulgare flowers, hepatoprotective activity, choleretic activity, antioxidant activity, subchronic toxiс hepatitis.


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