Comparative in vitro anti-aging activities of Phyllanthus emblica L. extract, Manilkara sapota L. extract and its combination.

Abstract

Keywords: Phyllanthus emblica L.; Manilkara sapota L.; Combination; Antioxidant; Anti-collagenase; Anti-   elastase

Objectives: To investigate in vitro anti-aging properties of Phyllanthus emblica L. (amla), Manilkara sapota L. (sapota) and its combination extracts including an in vitro antioxidant, anti-collagenase and anti-elastase activities for cosmetic application.

Methods: The ethanolic extracts of both individual amla and sapota extracts and combination extract at the ratios of 1:1 were prepared. The antioxidant capacity was investigated by DPPH radical scavenging assay. Anti-collagenase and anti-elastase assays properties were evaluated by using the EnzCheck^® assay kits (Molecular-Probes, Eugene, OR).

Results: The result of DPPH scavenging assay exhibited that amla showed the most potent activity (IC₅₀ 1.84±0.1 µg/ml) while sapota showed the weakest activity (IC₅₀ 29.7±129 µg/ml). The combination extract provided activity comparable to standard ascorbic acid and individual amla extract with IC₅₀ of 3.13±0.06 µg/ml. Conversely, strongest inhibitions against collagenase and elastase were detected for sapota with IC₅₀ of 65.68±3.63 and 36.82±0.72 µg/ml, respectively, followed by combination extract and individual amla extract.

Conclusion: Amla extract showed potent antioxidant activity while sapota showed potent anti-collagenase and anti-elastase activity. The combination of both extracts did not offer additive effect but rather synergistic effect which resulted in the strong antioxidant, anti-elastase and anti-collagenase activities. This suggested that this combination extracts is of interest for being use as cosmetic ingredient.