Chemical Compounds and Antioxidation Efficiency of Livistona speciosa Kurz. Seed Crude Extract

Abstract

Keywords: Livistona  speciosa  Kurz.; Antioxidant; Phenolic; Flavonoid; Tannin

Objectives: This research aimed 1) to study the amounts of  total phenolic, total tannin and total flavonoid contents in Livistona speciosa Kurz. seed crude extracts, 2) to separate the chemical compounds of Livisosa  speciosa  Kurz. seed crude extracts via chromatography technique, and 3) to study the antioxidant efficiency of crude extract from Livistona  speciosa  Kurz. seeds. 

Methods: Livistona  speciosa  Kurz. seed  crude extract was obtained by maceration technique using ethanol as a solvent. The crude extract was analyzed by UV-Visble spectrophotometer for the total phenolic content, the total tannin content and the total flavonoid content. The chemical compounds from crude extract were separated by column chromatography. The antioxidant efficiency of Livistona  speciosa  Kurz. seed crude extract and its chemical compounds were tested using DPPH radical scavenging assay.

Results: The results showed that the total phenolic content was 2.35 mg of gallic acid/ 1g of sample, the total tannin content was 4.26 mg of tannic acid/ 1g of sample and the total flavonoid content was 39.27 mg of rutin/ 1g of sample. Livistona  speciosa  Kurz. seed crude extract was generally separated into 5 fractions. Livistona  speciosa  Kurz. seed crude extract showed antioxidant activity with EC₅₀ as followed,13.50 x 10^-2, 2.59 x 10^-2, 17.73 x 10^-2, 4.09 x 10^-2 and 28.43 x 10^-2 mg/mL, respectively. While, EC₅₀ of BHT was 0.83 mg/mL.

Conclusion: From this study, It was found that Livistona  speciosa  Kurz. seed crude extract contained the high amount of flavonoid contents. Moreover, the Livistona  speciosa  Kurz. seed crude extract and the separated compounds consisted good value for antioxidant activity. Therefore, the crude extract of Livistona  speciosa  Kurz. seed crude extract can be further developed for healthy product or cosmetics.