Monodora myristica Seed Extracts ameliorate Crude Oil Contaminated Diet-induced cardio-toxicity in Rats

Abstract

This study investigated the ameliorative effects of Monodora myristica seed extracts in serum and heart of rats fed crude petroleum oil polluted diet (CPOPD). Thirty adult Wistar rats (180±5g) were used for the study. They were divided into six groups and treated as follows: Group A (normal control), Group B (crude petroleum oil polluted diet (CPOPD) control), Group C (CPOPD plus 1 ml/kg Tween 80), Group D (CPOPD plus 200 mg/kg M. myristica aqueous extract), Group E (CPOPD plus M. myristica ethanol extract, 200 mg/kg), Group F (CPOPD plus M. myristica diethyl ether extract, 200 mg/kg). The CPOPD and extracts were prepared following standard methods and administered for a period of 28 days after which the animals were sacrificed and tissues processed for biochemical and histological examinations. Exposure of rats to CPOPD significantly increased (p<0.05) the activities of lactate dehydrogenase (LDH) and Glucose -6-phosphate dehydrogenase (G6PDH) and the level of lipid peroxidation, but significantly decreased the activities of Glutathione peroxidase (GPx), Glutathione reductase (GR), Superoxide dismutase (SOD) and Catalase (CAT) compared to rats in the control group. In addition, histological analysis of the heart showed considerable necrosis and degeneration of cardiac cells in CPOPD-exposed rats compared to control.