Eriodictyol attenuates hydrogen peroxide-induced cell death in neuronal SH-SY5Y cells

Saowarose Thongin, Narudol Teerapattarakan, Hattaya Benya-aphikul, Varisa Pongrakhananon, Boonchoo Sritularak, Ratchanee Rodsiri

Abstract


Eriodictyol isolated from Dendrobium ellipsophyllum provides health benefits in several oxidative stress-related diseases. This study aimed to determine the protective effect and mechanisms of eriodictyol on hydrogen peroxide (H2O2)-induced cell death in neuronal SH-SY5Y cells. The protective effect of eriodictyol against H2O2 was evaluated by resazurin assay. Reactive oxygen species (ROS) and the mitochondrial membrane potential were detected by 2´-7´ dichlorodihydrofluorescein diacetate (DCFH-DA) and tetramethylrhodamine ethyl ester (TMRE) assays, respectively. Effects of eriodictyol on antioxidant enzyme expression and protein-associated apoptosis were determined using Western blot analysis. Pretreatment with eriodictyol suppressed cell death mediated by H2O2. Intracellular ROS and the loss of mitochondrial membrane potential induced by H2O2 were significantly reduced following eriodictyol treatment. Western blot analysis revealed that H2O2 enhanced the expression of, cytochrome c, cleaved caspase-3 and caspase-3 while H2O2 decreased the expression of Bcl-2/BAX, which was in turn attenuated in the presence of eriodictyol. Furthermore, pretreatment with eriodictyol extensively promoted the expression of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT), indicating the significant neuroprotective effect of eriodictyol through an antioxidant-dependent mechanism. Our data provide scientific information for the development of potent therapeutic agents for neurodegenerative diseases.

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References


Wang X, Wang W, Li L, et al. Oxidative stress and mitochondrial dysfunction in Alzheimer's disease. Biochim. Biophys. Acta 2014; 1842:1240 -1247.

Andersen JK. Oxidative stress in neurodegeneration: cause or consequence?. Nat. Med 2004; 10:S18 -25.

Kim GH, Kim JE, Rhie SJ, et al. The Role of Oxidative Stress in Neurodegenerative Diseases. J. Neuropathol. Exp. Neurol 2015; 24:325 -340.

Blesa J, Trigo-Damas I, Quiroga-Varela A, et al. Oxidative stress and Parkinson's disease. Front Neuroanat 2015; 9:1 -9.

Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol 2007; 39:44 -84.

Sies H. Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress. Redox Biol 2017; 11:613 -619.

Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress. Curr. Biol. 2014; 24:453 -462.

Whittemore ER, Loo DT, Watt JA, et al. A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture. J Neurosci 1995; 67:921 -932.

Zhou L, Yao GD, Song XY, et al. Neuroprotective Effects of 1,2-Diarylpropane Type Phenylpropanoid Enantiomers from Red Raspberry against H2O2-Induced Oxidative Stress in Human Neuroblastoma SH-SY5Y Cells. J. Agric. Food Chem 2018; 66:331 -338.

Tanagornmeatar K, Chaotham C, Sritularak B, et al. Cytotoxic and anti-metastatic activities of phenolic compounds from Dendrobium ellipsophyllum. Anticancer Res 2014; 34:6573 -6579.

Lv P, Yu J, Xu X, et al. Eriodictyol inhibits high glucose-induced oxidative stress and inflammation in retinal ganglial cells. J. Cell. Biochem 2019; 120:5644 -5651.

Wang Y, Chen Y, Chen Y, et al. Eriodictyol inhibits IL-1β-induced inflammatory response in human osteoarthritis chondrocytes. Biomed. Pharmacother 2018; 107:1128 -1134.

Sasaki N, Toda T, Kaneko T, et al. Protective effects of flavonoids on the cytotoxicity of linoleic acid hydroperoxide toward rat pheochromocytoma PC12 cells. Chem. Biol. Interact 2003; 145:101 -116.

Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv 2015; 5:27986 -28006.

Mattson MP. Apoptosis in neurodegenerative disorders. Nat. Rev. Mol. Cell Biol 2000; 1:120 -129.

Kwon SH, Ma SX, Lee SY, et al. Sulfuretin inhibits 6-hydroxydopamine-induced neuronal cell death via reactive oxygen species-dependent mechanisms in human neuroblastoma SH-SY5Y cells. Neurochem. Int 2014; 74:53 -64.

Suematsu N, Hosoda M, Fujimori K. Protective effects of quercetin against hydrogen peroxide-induced apoptosis in human neuronal SH-SY5Y cells. Neurosci. Lett 2011; 504:223 -227.

Chanput W, Krueyos N, Ritthiruangdej P. Anti-oxidative assays as

markers for anti-inflammatory activity of flavonoids. Int Immunopharmacol 2016; 40:170 -175.

Pavelescu LA. On reactive oxygen species measurement in living systems. J Med Life 2015; 8:38 –42.

Johnson J, Maher P, Hanneken A. The flavonoid, eriodictyol, induces long-term protection in ARPE-19 cells through its effects on Nrf2 activation and phase 2 gene expression. Invest. Ophthalmol. Vis. Sci 2009; 50:2398 -2406.

Sullivan PG, Brown MR. Mitochondrial aging and dysfunction in Alzheimer's disease. Prog. Neuropsychopharmacol. Biol. Psychiatry 2005; 29:407 -410.

Alvarino R, Alonso E, Tribalat MA, et al. Evaluation of the Protective Effects of Sarains on H2O2-Induced Mitochondrial Dysfunction and Oxidative Stress in SH-SY5Y Neuroblastoma Cells. Neurotox Res 2017; 32:368 -380.

Waseem M, Sahu U, Salman M, et al. Melatonin pre-treatment mitigates SHSY-5Y cells against oxaliplatin induced mitochondrial stress and apoptotic cell death. PloS one 2017; 12:1 -28.

Mattson MP. Apoptosis in neurodegenerative disorders. Nat. Rev. Mol. Cell Biol 2000; 1:120 -129.

Feng C, Luo T, Zhang S, et al. Lycopene protects human SHSY5Y neuroblastoma cells against hydrogen peroxideinduced death via inhibition of oxidative stress and mitochondriaassociated apoptotic pathways. Mol Med Rep 2016; 13:4205 -4214.


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