Cell lysis methods and coenzyme Q10 production of Methylobacterium strains

Abstract

Objective: This research aimed to investigate the effect of cell lysis methods on a quantity of coenzyme Q₁₀ (CoQ₁₀) produced by Methylobacteriumstrains. Methods: Methylobacterium organophilum NBRC 15689^T and Methylobacterium strain LRY1-08 were used to produce of CoQ₁₀. The 4 methods of cell lysis treatment were used to disrupt the cell wall of bacteria and to increase the release of CoQ₁₀ which was followed by extraction with isopropanol and hexane (3:5). Content of CoQ₁₀ was analyzed with HPLC method. Results: The results showed that the cell lysis treatment using methanol and 0.3% sodium chloride (10:1) containing 1% Triton X-100 with sonication and then extraction with isopropanol and hexane (3:5) were found to yield the highest CoQ₁₀ content for both Methylobacteriumstrains. Methelobacterium organophilum NBRC 15689^T produced significantly higher CoQ₁₀ content than Methylobacterium strain LRY1-08 which was consistent with its higher dry cell weight. Conclusion: The results indicated that this cell lysis method could be used to extract CoQ₁₀ from plasma membrane of the bacteria. Methylobacterium organophilum NBRC 15689^T was a promising strain for CoQ₁₀production source by fermentation which should be further optimized factors affecting CoQ₁₀ production.