Stability-Indicating Method to Determine Bioactive Nucleosides in Crude Drugs, Extracts, and products from Cordyceps Sinensis and Cordyceps Militaris
Abstract
The aim of this study was to develop a stability indicating method to determine bioactive nucleosides including uridine, guanosine, adenosine, and cordycepin in crude drugs, extracts, and products from Cordyceps Sinensis and Cordyceps Militaris by gradient reversed-phase HPLC. The C8 column (250mm x 4.6 mm id 5 µm) was used and the mobile phase was a mixture of water (A) and acetonitrile (B). The system was: 0-15 min, 1% B; 15-30 min, 1-15% B. The flow rate was 1 mL/min and the injection volume was 10 µl with UV detection at 254 nm. The correlation coefficients of linearity were more than 0.9995 for uridine (0.56-11.20 µg/ml), guanosine (0.56-11.21 µg/ml), adenosine (1.13-11.30 µg/ml) and cordycepin (0.279-2.793 µg/ml). The intra-day and inter-day precisions were less than 2% and 3%, respectively. The accuracy of the method was in the range of 96.65-100.64%. The studied nucleosides were degraded more in 0.1 N H2SO4 and 3%H2O2 than 0.1 N NaOH, sunlight, and heat at 90°C. The developed method was found to be specific to uridine, guanosine, adenosine, and cordycepin in the presence of sample matrices and their degradation products and could be applied to assess stability of crude drugs, extracts, and products from Cordyceps Sinensis and Cordyceps Militaris.
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