Anticonvulsant activity of Fumaria schleicheri dry extract and sodium valproate: role of neurotrophin and cytokine pathways
Abstract
The role of neurotrophin and cytokine link in anticonvulsant activity of Fumaria schleicheri dry extract and sodium valproate was assessed by the change in the cerebral level of nerve growth factor (NGF), interleukin 1β (IL-1β), interleukin 4 (IL-4) and tumor necrosis factor-α (TNF-α).
A total of 51 mice were divided into 4 groups: intact control, pentylenetetrazole, pentylenetetrazole + F. schleicheri dry extract, pentylenetetrazole + sodium valproate. F. schleicheri dry extract (100 mg/kg) and sodium valproate (300 mg/kg) were administered into the stomach once a day for 3 days. Pentylenetetrazole (80 mg/kg) was administered subcutaneously on the 3rd day, 30 minutes after the introduction of the tested drugs. After 18 hours the content of cerebral NGF and neurotropic cytokines was determined in the surviving animals.
Both Fumaria schleicheri dry extract and sodium valproate provide an expressed anticonvulsant activity, resulting in a significant improvement of investigated indicators of experimental seizures.
Pentylenetetrazole caused considerable changes in both neurotrophin NGF, and cytokines – IL-1β, TNF-α и IL-4. F. schleicheri dry extract as sodium valproate, decreased pentylenetetrazole-associated NGF overexpression, normalized the content of IL-1β, TNF-α and increased the level of IL-4. Correlation analysis proved the strong connection between NGF and cytokines in mice brain.
A total of 51 mice were divided into 4 groups: intact control, pentylenetetrazole, pentylenetetrazole + F. schleicheri dry extract, pentylenetetrazole + sodium valproate. F. schleicheri dry extract (100 mg/kg) and sodium valproate (300 mg/kg) were administered into the stomach once a day for 3 days. Pentylenetetrazole (80 mg/kg) was administered subcutaneously on the 3rd day, 30 minutes after the introduction of the tested drugs. After 18 hours the content of cerebral NGF and neurotropic cytokines was determined in the surviving animals.
Both Fumaria schleicheri dry extract and sodium valproate provide an expressed anticonvulsant activity, resulting in a significant improvement of investigated indicators of experimental seizures.
Pentylenetetrazole caused considerable changes in both neurotrophin NGF, and cytokines – IL-1β, TNF-α и IL-4. F. schleicheri dry extract as sodium valproate, decreased pentylenetetrazole-associated NGF overexpression, normalized the content of IL-1β, TNF-α and increased the level of IL-4. Correlation analysis proved the strong connection between NGF and cytokines in mice brain.
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