Evaluation of volatile constituents of fresh and dried herbals in Thai traditional compress ball by Head-Space GC-MS technique:(TJPS-2020-0057.R2)

Abstract

Introduction: There are two types of herbal compress balls, fresh and dried, which are normally used in combination with Thai traditional massage for relieving inflammation and pain. There is no comparing information about chemical compositions of these herbal compress balls. Objective: This research focused on analysis of chemical composition of two types of herbal compress balls by using headspace (HS) gas chromatography-mass spectrometry (GC–MS) system at a temperature of 80 ℃. Methods: Each herbal compress samples, Cryptolepis dubia, Cymbopogon citratus, Tamarindus indica. Curcuma longa, Zingiber montanum, and Citrus hystrix was placed in a headspace vial at 80 °C for 20 min, agitated at 500 rpm, and injected with splitless mode (1:10). The GC system was interfaced with Agilent 5975C inert XL EI/CI MSD at a mass range of 40.0−900.0 amu. The volatile profiles were evaluated by Principle Component Analysis (PCA). Results: Freshly prepared and dried compress balls materials showed 53 volatile compounds. Some parts of herbal materials gave more amounts of compound in fresh materials, but some dried part materials gave a greater number of compounds than fresh parts. Conclusion: Parts of the plant matrix play an important role in the amounts and number of volatile compounds.

:TJPS-2020-0057.R2