EFFECTS OF THE STANDARDIZED EXTRACT OF CENTELLA ASIATICA ECa233 ON HEPATIC PHASE II DRUG METABOLIZING ENZYMES IN RATS

Somsong Tuntaterdtum Lawanprasert

Abstract


The standardized extract of C. asiatica ECa233 (ECa 233) was shown to possess neuroprotective and wound healing effects. The purpose of this study was to investigate effects ECa 233 on the activities of phase II drug metabolizing enzymes including UDP-glucuronosyltransferase (UDPGT), sulfotransferase (SULT), glutathione S-transferase (GST) and NAD(P)H quinone oxidoreductase (NQOR) in vivo in rats. ECa 233 was orally given to both male and female Wistar rats at doses of 10, 100 and 1,000 mg/kg/d for 90 days. The results showed that all dosage regimens of ECa 233 did not affect the activities of UDPGT in both male and female rats. Furthermore, in line with our previous study in male rats, it was found that neither the activities of GST nor NQOR in female rats was modulated by ECa 233. However, the activity of SULT, which had been found to significantly decrease in male rats, was unaltered in female rats. The results obtained provide the proof of evidence of safety of ECa 233 on phase II drug-metabolizing enzymes in animals, then interpretation of these data from animals to human remains to be further investigated.

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References


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