Moss Bryum weigelii Spreng improves survival in septic rats induced by cecal ligation and puncture:(TJPS-2020-0165.R2)

Abstract

The present study investigated the phytochemical, HPTLC profiling, total phenolic content, antibacterial activity, acute toxicity studies, hemodynamic parameters, myeloperoxidase activity and serum procalcitonin levels of hydroalcoholic extract of Bryum weigelii (HE-Bw), and its effects on cecal ligation and puncture (CLP) induced sepsis in mice. The preliminary phytochemical screening showed that HE-Bw contains alkaloids, terpenoids, flavonoids, phenolics, and tannins. The HPTLC analysis of HE-Bw identified three unknown components with different Rf values and area percentage. The total phenolic amount for HE-Bw was equivalent to 211.6±0.8 mg of gallic acid per 100 g of dried plant material. At 100 µg/ml concentration, HE-Bw showed antibacterial activities as potent as streptomycin against Staphylococcus aureus and Escherichia coli. Acute toxicity studies on mice found out that HE-Bw was non-toxic up to 2000 mg/Kg body weight. At both low and high doses, HE-Bw improved hemodynamic parameters such as mean arterial pressure and decreased optical density of blood, while decreased serum myeloperoxidase activity and procalcitonin levels. Moreover, at a high dose, HE-Bw showed a survival rate of 92.50±3.50% in mice that might be through proinflammatory and bacteremia effects. The results indicate that B. weigelii can be a favorable natural source for CLP-induced sepsis treatment in mice.

:TJPS-2020-0165.R2