High-performance thin-layer chromatography analysis of (E)-4-(3ʹ,4ʹ-dimethoxyphenyl)but-3-en-1-ol (compound D) in Zingiber cassumunar Roxb rhizome extract: An application on genetic differentiation

Prasan Tangyuenyongwatana, Piyarat Parinyapong Chareonsap, Wandee Gritsanapan

Abstract


Objectives: This study focused on developing high performance thin-layer chromatography (HPTLC) quantitative analysis method for compound D, a major anti-inflammatory compound in Zingiber cassumunar extract. In addition, the amplified fragment length polymorphism (AFLP) technique was applied to explore the genetic differentiation relating to the yield of compound D. Methods: HPTLC method validation included linearity, precisions, accuracy, limit of detection (LOD), limit of quantitation (LOQ) and robustness was performed according to ICH guideline. For genetic differentiation in Z. cassumunar, AFLP method was applied to this study. Results: Linearity of the developed method showed a regression coefficient at 0.9958 and covered concentration range of 84 - 420 ng/band. The precisions were less than 2% (RSD). The accuracy demonstrated the average recovery value of 98.31%. The LOD and LOQ were 10 and 40 ng.  The results on robustness of the method were also less than 2% (RSD). When applied this method to determine the amounts of compound D in Z. cassumunar rhizome extracts from 17 locations in Thailand, the results indicated some correlation between amount of compound D and major genetic differentiation in Z. cassumunar. Conclusion: The validated HPTLC method has high potential in application of quality control of compound D in raw material of different variety of Z. cassumunar rhizomes, and its herbal pharmaceutical products.  


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References


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