Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on TNF-α and IL-6 cytokine balance of the mouse macrophage cell lines

Theeraya Krisdaphong

Abstract


Objectives: The aim of this investigation was to create value to the industrial waste production by systematic extraction and purification of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material. In addition, the immunological activity of the obtained β-glucan was also evaluated. Methods: The β-glucan from the yeast waste material of the brewing production was isolated by cell autolysis fractioning, solvent extraction and drying. The obtained β-glucan was characterized by HPLC, NMR and comparison with information in the literature. The characterized β-glucan was then examined for its immunomodulatory activity on RAW 264.7 cell. Results: The optimal extraction method of β-glucan from the brewing waste material showed high extraction yield (13.14% w/w). In addition, the immunomodulatory study on RAW 264.7 cell lines showed that the isolated β-glucan could suppress the TNF-α and IL-6 production in the cell lines at 6 h and 24 h, compared to the LPS control.


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