Inhibitory effects of Manosa alliacea in Freund's adjuvant arthritis on inflammatory markers and its confirmation by In-silico strategy:(TJPS-2021-0103.R1)

Abstract

For the assessment of the curative effect of M. alliacea on Freud's adjuvant arthritis (FA) on Swiss albino rats. Methanol extract from M. alliacea (MEMA) administered orally at 200 mg/kg and 400 mg/kg, 28 days after FA immunization. For control and treatment groups, paw volume, body weight, haematological parameters, x-ray and histological tests were measured. In addition, RT-PCR was used to measure the levels of various inflammatory markers. In vitro, DPPH and H2O2 tests were used to evaluate the antioxidant capability. MEMA decreased paw volume and paw thickness, bodyweight considerably (p<0.05, 0.01, 0.001), compared to haematological anomalies of arthritis control. X- rays tests and histological tests did not reveal significant structural changes in the rat ankle joints administered with MEMA. The levels of expression TNF - α, NF-Kβ, IL-1β and COX-2 were significantly suppressed in the treatment groups. The in-silico study has shown that a number of chemical components in the plants under study can effectively bind to various inflammatory targets. That is why we say M. alliacea is a good source for treating rheumatoid arthritis.