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Comparative evaluation of neuroprotective effect of three varieties of Allium cepa in chronic constriction injury induced neuropathic pain

Amit Kumar, Kundan Singh Bora, Amteshwar Singh Jaggi, Richa Shri

Abstract


Objectives: Allium cepa has been used extensively for culinary purposes as well as in traditional system of medicine. A number of varieties of A. cepa are available commercially with varietal differences in phytoconstituents and biological activities. However their effect on neuropathic pain has not been explored. The present study was designed to compare the effectiveness of three varieties of A. cepa in neuropathic pain. Materials & Methods: Methanol extracts and flavonoid-rich fractions (FRF) of outer scales of three varieties of A. cepa viz. Agrifound Dark Red (ADR), Agrifound White (AW) and NHRDF-Red (L28) were prepared. These were standardized with reference to their total phenol and flavonoid content. The marker- quercetin was determined by High Performance Thin Layer Chromatography (HPTLC). The effect of the prepared extracts was studied on neuropathic pain (NP) induced by chronic constriction injury (CCI) in rats. Behavioral indices of sensory dysfunction due to NP were evaluated by studying various behavioral tests viz. pin prick test, Eddy’s hot plate test and Randall-Selitto test for hyperalgesia, and acetone drop test for allodynia. Markers of oxidative stress were studied by determining Thio-barbituric Acid Reactive Species (TBARS) and reduced glutathione (GSH) levels. Results: FRF of variety L28 produced the most significant reduction of thermal and mechanical hyperalgesia and amelioration of cold allodynia. This also attenuated the CCI-induced increase in TBARS and decrease in GSH levels. Conclusion: L28 was found to contain highest amount of flavonoids and quercetin as compared to other varieties. These results indicate that FRF of A. cepa variety L28 may be a potential candidate for the management of NP.

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References


H. Merskey, N. Bogduk. A. S. P. Taxonomy updated from "Part III: Pain Terms, A Current List with Definitions and Notes on Usage" Classification of Chronic Pain, second Edition, I.A.S.P. Task Force on Taxonomy, IASP Press, Seattle 1994; 209-214.

A. S. Jaggi, N. Singh. Differential effect of spironolactone in chronic constriction injury and vincristine-induced neuropathic pain in rats. Eur. J. Pharmacol. 2010, 648, 102-109.

P. Honore, M. F. Jarvis. Acute and Neuropathic Pain, in: Editors-in-Chief: B. T. John, J. T. David, (Eds.), Comprehensive Medicinal Chemistry II. Elsevier, Oxford. 2007, pp. 327-349.

M. Colleoni, P. Sacerdote. Murine models of human neuropathic pain. Biophysica. Acta. 2010, 1802, 924-933.

K. E. Galluzzi. Managing Neuropathic Pain. J. A. O. A, J. Am. Osteopath Assoc. 2007, 107: ES39-ES48.

R. H. Dworkin, A. B. O. Connor, J. Audette, R. Baron, G. K. Gourlay, M. L. Haanpaa, J. L. Kent, E. J. Krane, A. A. LeBel, R. M. Levy, S. C. Mackey, J. Mayer, C. Miaskowski, S. N. Raja, A. S. C. Rice, K. E. Schmader, B. Stacey, S. Stanos, R. D. Treede, D. C. Turk., G. A. Walco, C. D. Wells. Recommendations for the Pharmacological Management of Neuropathic Pain. An Overview and Literature Update, Mayo Clinic. Proceedings, 2010, 85, S3-14.

S. B. Rafnsson, V. Dilis, A. Trichopoulou. Antioxidant nutrients and age-related cognitive decline: a systematic review of population-based cohort studies. Eur. J. Nutr. 2013, 52, (6): 1553-67.

M. L. Wahlqvist. Antioxidant relevance to human health. Asia Pac. J. Clin. Nutr. 2013, 22, (2), 171-6.

V. Tiwari, A. Kuhad, K. Chopra. Amelioration of functional, biochemical and molecular deficits by epigallocatechingallate in experimental model of alcoholic neuropathy. Eur. J. Pain. 2011, 15, (3), 286-92.

A. D. Kandhare, K. S. Raygude, P. Ghosh, A. E. Ghule, S. L. Bodhankar. Neuroprotective effect of naringin by modulation of endogenous biomarkers in streptozotocin induced painful diabetic neuropathy. Fitoterapia, 2012, 83, (4), 650-9.

J. Zhang, C. Lv, H. N. Wang, Y. Cao. Synergistic interaction between total glucosides and total flavonoids on chronic constriction injury induced neuropathic pain in rats. Pharm. Biol., 2013, 51, (4), 455-62.

A. W. Boots, G. R. Haenen, A. Bast. Health effects of quercetin: from antioxidant to nutraceutical. Eur. J. Pharmacol. 2008, 585, (2-3), 325-37.

E. J. Yang, G. S. Kim, J. A. Kim, K. S. Song. Protective effects of onion-derived quercetin on glutamate-mediated hippocampal neuronal cell death. Pharmacogn. Mag., 2013, 9, (36), 302-308.

S. Kapoor. Systemic alcohol-protective effects of quercetin besides its ameliorating effect on alcohol-induced neuropathic pain, Inflammopharmacol. 2012, 20, (5), 295.

K. S. Raygude, A. D. Kandhare, P. Ghosh, A. E. Ghule, S. L. Bodhankar. Evaluation of ameliorative effect of quercetin in experimental model of alcoholic neuropathy in rats, Inflammopharmacol. 2012, (6), 331-41.

F. Dajas. Life or death: neuroprotective and anticancer effects of quercetin. J. Ethnopharmacol. 2012, 143, (2), 383-96.

R. Slimestad, T. Fossen, I. M. Vågen. Onions: a source of unique dietary flavonoids. J. A. Gric. Food Chem. 2007, 55, (25), 10067-80.

B. N. Singh, B. R. Singh, R. L. Singh, D. Prakash, D. P. Singh, B. K. Sarma, G. Upadhyay, H. B. Singh. Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. Food Chem. Toxicol. 2009, 47, 1161-1167.

G. Singh. Onion, in Checklist on commercial Varieties of vegetables. Department of Agriculture & Cooperation, Ministry of Agriculture, Government of India, New Delhi, 2012; 37-42.

B. Patil, L. Pike. Distribution of quercetin content on different rings of various onion cultivars. J. Hortic. Sc. 1995, 70, 643-650.

J. Yang, J. K. Meyers, J. van der Heide J, R. H. Liu. Varietal differences in phenolic content and antioxidant and antiproliferative activities of onions. J. Agric. Food Chem. 2004, 52, (22), 6787-93.

G. Griffiths, L. Trueman, T. Crowther, B. Thomas, B, Smith. Onions-a global benefit to health. Phytother. Res. 2002, 6, (7), 603-15.

K. S. Bora, A. Sharma. Phytoconstituents and Therapeutic Potential of Allium cepa Linn– A Review. Pharmacogn. Rev, 2009, 3, (5), 159-169.

H. Y. Fu. Free Radical Scavenging and Leukemia Cell Growth Inhibitory Properties of Onion Powders Treated by Different Heating Processes. J. Food Sci. 2004, 69,SNQ50-SNQ54.

S. Park, M-Y. Kim, D. Lee, D. Lee, E. Baik, C-H. Moon, S. Park, E. Ko, S-R. Oh, Y-S. Jung. Methanolic extract of onion (Allium cepa) attenuates ischemia/hypoxia-induced apoptosis in cardiomyocytes via antioxidant effect. Eur. J. Nutr. 2009, 48, 235-242.

K. Kumari, K. T. Augusti. Lipid lowering effect of S-methyl cysteine sulfoxide from Allium cepa Linn in high cholesterol diet fed rats. J. Ethnopharmacol. 2007; 109, 367-371.

H. D. Brahmachari, K. T. Augusti. Effects of orally effective hypoglycaemic agents from plants on alloxan diabetes. J. Pharm. Pharmacol.1962, 14: 617.

G. Saravanan, P, Ponmurugan. Ameliorative potential of S-allylcysteine: Effect on lipid profile and changes in tissue fatty acid composition in experimental diabetes. Exp. Toxicol. Pathol. 2012, 64, 639-644.

J. Sanderson, W. R. McLauchlan, G. Williamson. Quercetin inhibits hydrogen peroxide-induced oxidation of the rat lens. Free Radic. Biol. Med. 1999, 26, 639-645.

A. N. Zohril, K. A. Gawadl, S. Saber. Antibacterial, antidermatophytic and antitoxigenic activities of onion. Microbiol. Res. 1995, 150, 167-172.

J. Santas, M. P. Almajano, R. Carbó. Antimicrobial and antioxidant activity of crude onion (Allium cepa, L.) extracts. Int. J. Food Sci. Tech. 2010, 45, 403-409.

R. Shri, K. S. Bora. Neuroprotective effect of methanolic extracts of Allium cepa on ischemia and reperfusion-induced cerebral injury. Fitoterapia, 2008, 79, 86-96.

C. Kaur, S. Joshi, H. C. Kapoor. Antioxidants in onion (Allium cepa) cultivars grown in India. J. Food Biochem. 2009, 33, (2), 184–200.

T. N. Ly, C. Hazama, M. Shimoyamada, H. Ando, K. Kato, R. Yamauchi. Antioxidative Compounds from the Outer Scales of Onion. J. Agric. Food Chem. 2005, 53, 8183-8189.

R. Madaan, G. Bansal, S. Kumar, A. Sharma. Estimation of total phenols and flavonoids in extracts of Actaea spicata roots and antioxidant activity studies. Indian. J. Pharm. Sci. 2011, 73, 666.

A. E. Hagerman, L. G. Butler. Protein precipitation method for the quantitative determination of tannins. J. Agric. Food Chem. 1978, 26, 809-812.

V. L. Singleton, R. Orthofer, R. M. Lamuela-Raventós. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent, in: Lester, P. Ed; Methods in Enzymology; Academic Press. 1999; 152-178.

M. Jay, J-F. Gonnet, E. Wollenweber, B. Voirin. Sur l'analyse qualitative des aglycones flavoniques dans une optique chimiotaxinomique. Phytochem. 1975, 14, 1605-1612.

T. J. Maves, P. S. Pechman, G. Gebhart, S. T. Meller. Possible chemical contribution from chromic gut sutures produces disorders of pain sensation like those seen in man. Pain.

S. Flatters, G. Bennett. Ethosuximide reverses paclitaxel- and vincristine-induced painful peripheral neuropathy. Pain. 2004, 109, 150-161.

H. Erichsen, G. Munro- Blackburn. Pharmacological characterisation of the spared nerve injury model of neuropathic pain. Pain. 2002, 98, 151-161.

V. Jain, A. Jaggi, N. Singh. Ameliorative potential of rosiglitazone in tibial and sural nerve transection-induced painful neuropathy in rats. Pharmacol. Res. 2009, 59, 385-392.

L. Randall, J. Selitto. A method for measurement of analgesic activity on inflamed tissue. Archives internationales de pharmacodynamie et de thérapie. 1957,111, 409-419.

O. H. Lowry, N. J. Rosebrough, A. L. Farr, R. J. Randall. Protein measurement with the Folin phenol reagent. J. Biol. Chem.1951,193, 265-275.

H. Ohkawa, N. Ohishi, K. Yagi. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979, 95, 351-358.

E. Beutler, O. Duron, B. Kelly. Improved method for the determination of blood glutathione. J. Lab. Clin. Med.1963, 61, 882-888.

V. Lanzotti. The analysis of onion and garlic. J. Chromatogr. 2006, 1112, (1-2), 3-22.

M-Y. Shon, S-D. Choi, G-G. Kahng, S-H. Nam, N-J. Sung. Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food Chem. Toxicol. 2004, 42, 659-666.

T. Albishi, J. A. John, A. Al-Khalifa, F. Shahidi. Antioxidative phenolic constituents of skins of onion varieties and their activities. J. Funct. Foods, 2013, 5, 1191-1203.

K. Babu, S. Garg, Mohapatra, K. P., Fernandez, X. P., Purohit, S. J. Comparison of nutritional values of different varieties of Onions cultivated in India. International J. Itegrative Siences, Inovation Tchnol. 2012, 1, (4), 25-31.

B. Patil, L. Pike, K. Yoo. Variation in the quercetin content in different colored onions (Allium cepa L.). J. Am. Soc. Hortic. Sc. 1995, 120, 909-913.

Y. Benmalek, O. A. Yahia, A. Belkebir, M. L. Fardeau. Anti-microbial and anti-oxidant activities of Illicium erum, Crataegus xyacantha spp monogyna and Allium cepa red and white varieties. Bioengineered. 2013, 4, (4), 244-8.

Y. A. Elhassaneen, M. I. Sanad. Phenolics, selenium, vitamin C, amino acids and pungency levels and antioxidant activities of two Egyptian onion varieties. Am. J. Food Tech. 2009, 4, (6), 241–254.

K. R. Price, M. J. C. Rhodes. Analysis of the Major Flavonol Glycosides Present in Four Varieties of Onion (Allium cepa) and Changes in Composition Resulting from Autolysis. J. Sci. Food Agric. 1997, 74, 331-339.

D. Caridi, V. C. Trenerry, S. Rochfort, S. Duong, D. Laugher, R. Jones. Profiling and quantifying quercetin glucosides in onion (Allium cepa L.) varieties using capillary zone electrophoresis and high performance liquid chromatography. Food Chem. 2007, 105, 691-699.

G. J. Bennett, Y-K. Xie. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain, 1988, 33, 87-107.

E-S. Park, X. Gao, J. M. Chung, K. Chung. Levels of mitochondrial reactive oxygen species increase in rat neuropathic spinal dorsal horn neurons. Neurosci. Lett. 2006, 391, 108-111.

N. Attal, G. Filliatreau, S. Perrot, F. Jazat, L. Di Giamberardino, G. Guilbaud. Behavioural pain-related disorders and contribution of the saphenous nerve in crush and chronic constriction injury of the rat sciatic nerve. Pain, 1994, 59, 301-312.

H. K. Kim, S. K. Park, J-L. Zhou, G. Taglialatela, K. Chunng, R. E. Coggeshall, J. M. Chung. Reactive oxygen species (ROS) play an important role in a rat model of neuropathic pain. Pain. 2004, 111, 116-124.

J. Yowtak, K. Lee, H. Kim, J. Wang, K. Chung, J. Chung. Reactive oxygen species contribute to neuropathic pain by reducing spinal GABA release. Pain, 2011, 152, 844-852.

M. Anjaneyulu, K. Chopra. Quercetin, a bioflavonoid, attenuates thermal hyperalgesia in a mouse model of diabetic neuropathic pain. Prog. Neuropsychopharmacol. Biol. Psychiatry. 2003, 27,1001-1005.

X-K. Fang, J. Gao, D-N. Zhu. Kaempferol and quercetin isolated from Euonymus alatus improve glucose uptake of 3T3-L1 cells without adipogenesis activity. Life Sci. 2008, 82, 615-622.

S-L. Yeh, C-L. Yeh, S-T. Chan, C-H. Chuang. Plasma rich in quercetin metabolites induces G2/M arrest by upregulating PPAR-γ expression in human A549 lung cancer cells. Planta. Med. 2011, 77, 992-998.

A. S. Jaggi, N. Singh. Therapeutic targets for the management of peripheral nerve injury-induced neuropathic pain. CNS Neurol. Disord. Drug Targets. 2011, 10, 589-609.


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