Effect of Ipomoea hederacea Jacq. methanolic extract on blood pressure and relaxation of rat thoracic aorta: evidence indicated the release of NO and H2S

chaweewan jansakul

Abstract


Seeds of Ipomoeahederacea Jacq. (IH) are used in traditional medicine to manage ailments pertaining to the vasculature, circulatory system and related disorders. However no study exists to validate their usage for said ailments. The present study was conducted to investigate the plant seed methanolic extract (IH extract) on blood pressure in an anesthetized rat and to elucidate the possible mechanisms on isolated thoracic aorta. The IH extract led to a decrease in blood pressure, and a relaxation of isolated thoracic aortic ring precontracted with phenylephrine. The relaxant effect was significantly inhibited by NG-nitro-L-arginine, ODQ, or removal of the endothelium, but not by glybenclamide or tetraethylammonium. The IH extract potentiated the relaxant effect of glyceryl trinitrate in a similar way to that of sildenafil with a further increase in maximal relaxation, the effect of which was significantly inhibited by DL-propargylglycine. An IH extract or Y-27632, a Rho-kinase inhibitor, each suppressed the concentration-response (C-R) curve of the phenylephrine on thoracic aorta, and when both were added this resulted in a total suppression. In normal Krebs solution, nifedipine caused no further inhibition of the phenylephrine C-R curve on thoracic aortic rings in the presence of the IH extract.These results indicate that the IH extract causes a relaxation of the thoracic aortic ring by stimulating the release of nitric oxide and H2S and might also act as an inhibitor of phosphodiesterase-5, a Rho-kinase and a voltage-gated Ca2+ channel, which then results in a lowering of the blood pressure in anesthetized rats.


Full Text:

58-65;PDF

References


Adnan M, Begum S, Khan AL, Tareen AM, Lee IJ. Medicinal plants and their uses in selected temperate zones of Pakistani Hindukush-Himalaya. J Med Plant Res 2012;6:4113-27.

Azad SA, Shah A. Some ethno-medicinal plants of district rajouri (Jammu province). Ind J Life Sci 2011;22:47-9.

Zia-Ul-Haq M, Riaz M, DeFeo V. Ipomoea hederaceaJacq.: A medicinal herb with promising health benefits. Molecules 2012b;17:13132-45.

Nam KA, Lee SK. Evaluation of cytotoxic potential of natural products in cultured human cancer cells. Nat Prod Sci2000;6:183–8.

Rehman A, Abbas A,Riaz T, Ahmad S, Abbasi A. Ipomoea hederacea: an imperative source for natural antioxidants. Asain J Pharm Bio Res2011;1:534-41.

Zia-Ul-Haq M, Ahmad S, Calani L, Mazzeo T, DelRio D, Pellegrini N, et al. Compositional study and antioxidant potential of Ipomoea hederacea Jacq. andLepidiumsativum L. seeds. Molecule2012a;17:10306-21.

Ruckmani DS, Chitra M, Jayamathi P. Hepatoprotectivity and an antioxidant study of Ipomoea hederacea on experimentally induced hepatotoxic rats. Rec Res Sci Tech 2010;2:17-9.

Ahmad M, Jabeen M, Zia-Ul-Haq M, Jahan N. Determination of LD50 and ED50 by dose response relationship and assessment of toxicological and non-toxicological behaviour of Ipomoea hederacea. J Pharm Res 2011;4:1176–8.

Zia-Ul-Haq M, Riaz M, Ahmad S, Barbes L, Velter V, Badiu D, et al. Anticonvulsant and sex enhancing effects of Ipomoea hederacea seeds extract. Farmacia 2014;62:737-42.

Lasker GF, Maley JH, Kadowitz PJ. A review of the

pathophysiology and novel treatments for erectile dysfunction. Adv PharmSci 2010;2010:730861.

Janbaz KH, Javed S, Saqib F, Imran I, Zia-Ul-Haq M, DeFeo V. Validation of ethnopharmacological uses of HeliotropiumstrigosumWilld. as spasmolytic, bronchodilator and vasorelaxant remedy. BMC Comp Alter Med 2015;15:169-75.

Jansakul C, Boura ALA, King RG. Effects of endothelial cell removal on constrictor and dilator responses of aortae of pregnant rats. J Autonomic Pharm1998;9:93-101.

Brayden JE. Functional roles of KATP channels in vascular smooth muscle. ClinExp Pharm Physiol 2002;29:312-6.

Ko EA, Han J, Jung ID, Park WS. Physiological roles of K+ channels in vascular smooth muscle cells. J Smooth Mus Res 2008;44:65–81.

Bhatia M. Hydrogen sulfide as a vasodilator. IUBMB Life 2005;57:603-6.

Streeter E, Hart J, Badoer E. An investigation of the mechanisms of hydrogen sulfide-induced vasorelaxation in rat middle cerebral arteries. NaunynSchmiedeberg Arch Pharmacol 2012;385:991-1002.

Sun Y, Tan CS, Jin HF, Du JB. The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings. Acta Pharm Sinica 2011;32:456-64.

Al-Magableh MR, Hart JL. Mechanism of vasorelaxation and role of endogenous hydrogen sulfide production in mouse aorta. NaunynSchmiedeberg Arch Pharmacol 2011;383:403-13.

Cheng Y, Ndisang JF, Tang G, Cao K, Wang R. Hydrogen sulfide-induced relaxation of resistance mesenteric artery beds of rats. Am J Physiol 2004;287:H2316-23.

Tang C, Li X, Du J. Hydrogen sulfide as a new endogenous gaseos transmitter in the cardiovascular system. Curr Vas Pharmacol 2006;4:17-22.

Carter RW, Begaye M, Kanagy NL. Acute and chronic NOS inhibition enhances α2-adrenoreceptor-stimulated Rho A and Rho kinase in rat aorta. Am J Physiol 2002;283: H1361-9.

Jalil J, Lavandero S, Chiong M, Ocaranza MP. Rho/Rho kinase signal transduction pathway in cardiovascular disease and cardiovascular remodeling. RevEspañCard 2005;58:951-61.

Ishizaki T, Uehata M, Tamechika I, Keel J, Nonomura K, Maekawa M, Narumiya S. Pharmacological properties of Y-27632, a specific inhibitor of Rho-associated kinases.Mol Pharmacol 2000;57:976-83.

Nagumo H, Sasaki Y, Ono Y, Okamoto H, Seto M, Takuwa Y. Rho kinase inhibitor HA-1077 prevents Rho-mediated myosin phosphatase inhibition in smooth muscle cells. Am J Cell Physiol 2000;278:C57-65.

Wirth A. Rho kinase and hypertension. BiochimBiophysActa2010;1802:1276-84.

Uehata M, Ishizuki T, Satoh H, Ono T, Kawahara T, Morishita T, et al. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature1997;389:990-4.

Noguera MA, Madrero Y, Ivorra MD, D'Ocon P. Characterization of two different Ca2+ entry pathways dependent on depletion of internal Ca2+pools in rat aorta. Naunyn-Schmiedeberg Arch Pharmacol1998;357:92-9.

Abebe W, Harris H, MacLeod KM. Enhanced contractile responses of arteries from diabetic rats to α1-adrenoreceptor stimulation in the absence and presence of extracellular calcium. J CardiovasPharmacol1990;16:239-48.

Akata T. Cellular and molecular mechanisms regulating vascular tone. Part 1: basic mechanisms controlling cytosolic Ca2+ concentration and the Ca2+-dependent regulation of vascular tone. J Anesthesia 2007;21:220-31.

Nelson MT, Standen NB, Brayden JE, Worley JF. Noradrenaline contracts arteries by activating voltage-dependent calcium channels. Nature 1998;336:382-5.


Refbacks

  • There are currently no refbacks.