The Antioxidant Activity of Red Ginger Oil in Aloxan-induced Painful Diabetic Neuropathy in Mice Model
Abstract
Background: Red ginger oil (RGO) was reported had the highest bio-constituent, especially its essensial oil which was important for antioxidant, than the other varieties. Chronic diabetic triggered the increasing of reactive oxygen species (ROS) which responsible for diabetic complication such as painful diabetic neuropathy (PDN). Aim of the Study: The aims of this study is evaluate the relationship between antioxidant activity of RGO with PDN condition in mice model. Material and Method: Two varieties of ginger i.e. red ginger and ginger, were destilated using water, then calculated total phenolic content and the antioxidant activity using DPPH. In PDN model, mice were induced PDN using aloxan 210 mg/kg BW i.p. After ten days, diabetic mice were showed hyperalgesia then randomized into 7 groups i.e. normal, diabetic, glibenclamide and RGO (with dose 100, 200, 400 and 600 mg/kg BW). Treatment were given once daily for 14 days. Hyperalgesia response was evaluated using hot plate as latency time. The latency time and fasting blood glucose levels were observed at day-0, 3, 10, 17 and 24. Result: RGO had higher total phenolic content (0.778±0.175 mg GAE/g oil) and antioxidant activity using DPPH (IC50 3.626±0.357 µl/ml) than ginger oil. In vivo evaluation using mice, showed that RGO 600 mg/kg BW had the best activity in reducing hyperalgesia and restoring spinal cord damage after PDN compared to diabetic group (p < 0.05). RGO also had slightly effect in reducing blood glucose level and repairing islet pancreas cell. Conclusion: The antihyperalgesia of red ginger oil in PDN was related into its strong antioxidant ability compared to the other varieties.
Key word: Ginger, Red ginger oil, antioxidant, painful diabetic neuropathy (PDN).
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World Health Organization (WHO). Noncommunicable diseases country profiles. Geneva : WHO Publishing; 2011.
World Health Organization (WHO). Definition, diagnosis and classification of diabetes melitus and it’s complications. Geneva: WHO Publishing; 2010.
Kawano T. A current overview of diabetic neuropathy – mechanisms, symptoms, diagnosis, and treatment, peripheral neuropathy. InTech 2014; 10(5): 89-105.
Farmer KL, Li C, Dobrowsky RT. Diabetic Peripheral Neuropathy: Should a Chaperone Accompany Our Therapeutic Approach? Pharmacol Rev 2012; 64: 880-900.
Luongo L, Costa B, D’Agostino B, Guida F, Comelli F, Gatta L, Matteis M, Sullo N, De Petrocellis L, de Novellis V, Maione S, Di Marzo V. Palvanil, a non-pungent capsaicin analogue, inhibits inflammatory and neuropathic pain with little effects on bronchopulmonary function and body temperature. Pharmacol Res 2012; 66: 243-250.
Zhuo M. Long-term potentiation in the anterior cingulate cortex and chronic pain. Philos Trans R Soc B 2013; 369: 20130146.
Hartemann A, Attal N, Bouhassira D, Dumont I, Gin H, Jeanne S, Said G, Richard J -L. Painful diabetic neuropathy: Diagnosis and management. Diabetes Metab 2011; 37: 377-388.
Herlina, Murhananto, Endah, Listyarini, Pribadi. Khasiat manfaat jahe merah si rimpang ajaib. Jakarta: Agro Media Pustaka; 2002.
Anfenan MLK. Evaluation of nutritional and antidiabetic activity of different forms of ginger in rats. Middle-East J of Scient Resc 2014; 21(1): 56-62.
Rialita T, Rahayu WP, Nuraida L, Nurtama B. Aktivitas antimikroba minyak esensial jahe merah (Zingiber officinale Var. Rubrum) Dan Lengkuas Merah (Alpinia purpurata K. Schum) terhadap bakteri patogen dan perusak pangan. Agritech 2015; 35(1): 1-8.
Fuentes, Báez, Bravo, Cid, Labra. Determination of total phenolic content in olive oil samples by uv–visible spectrometry and multivariate calibration. Food Analytical Methods 2012; 5(6): 1311-1319.
Singh, Kapoor, Singh, de Heluani, de Lampasona, Catalan. Chemistry, antioxidant, and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food and Chemical Toxicology 2008; 46(10): 3295-3302.
Deshpande, Une, Lahoti, Dehghan. Protective effect of ethyl acetate soluble fraction of ethanolic extract of terminalia chebula retz, fruits on diabetic neuropathy in mice. Euro J Exp Bio 2011; 1(2): 139-14.
Fong SW, Lin H -C, Wu M -F, Chen C -C, Huang Y -S. CPEB3 Deficiency Elevates TRPV1 Expression in Dorsal Root Ganglia Neurons to Potentiate Thermosensation. PLOS ONE 2016; 11:e0148491.
Nash MS, McIntyre P, Groarke A, Lilley E, Culshaw A, Hallett A, Panesar M, Fox A, Bevan S. 7-tert-Butyl-6-(4-Chloro-Phenyl)-2-Thioxo-2,3-Dihydro-1H-Pyrido[2,3-d]Pyrimidin-4-One, a Classic Polymodal Inhibitor of Transient Receptor Potential Vanilloid Type 1 with a Reduced Liability for Hyperthermia, Is Analgesic and Ameliorates Visceral Hypersensitivity. J Pharmacol Exp Ther 2012; 342: 389-398.
Mohini A, Phansea SB, Padhyeb MJ, Patila AR, Bafnaa AR, Takawalec VV. Thespesia populnea extract attenuates thermal hyperalgesia in diabetic mouse model of neuropathic pain. IJPS Autumn 2010; 6(4): 269-276.
Dobretsov M, Romanosky D, dan Stimer JR. Early diabetic neuropathy: trigger and mechanisms. World J Gastroenterol 2007; 13(2):175-191.
Deshpande, Une, Lahoti, Dehghan. Protective effect of ethyl acetate soluble fraction of ethanolic extract of terminalia chebula retz, fruits on diabetic neuropathy in mice. Euro J Exp Bio 2011; 1(2): 139-14.
Bhatti, Rawal, Singh, Ishar. Effect of Aegle marmelos leaf extract treatment on diabetic neuropathy in rats: a possible involvement of α2 adrenoceptors. International Journal of Pharmacy and Pharmaceutical Science 2012; 4(3): 632-637.
Heimes K, Feistel B, Verspohl EJ. Impact Of The 5-HT3 Receptor Channel System For Insulin Secretion And Interaction Of Ginger Extracts. European Journal of Pharmacology 2009; 624(3): 58-65.
Baroty, Baky, Farag, Saleh. Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils. African J Biochem Res 2010; 4(6): 167-174.
Basak SS, Candan F. Chemical composition and in vitro antioxidant and antidiabetic activities of eucalyptus camaldulensis dehnh essential oil. J Iran Chem Soc 2010; 7(1): 216-226.
Kumari, Venkateshwarlu, Choukse, Anandan. Effect of essential oil and aqueous extract of ginger (zingiber officinale) on oxidative stability of fish oil-in-water emulsion. J Food Process Technol 2014; 6(1): 1-5.
Tiwari M, Kakkar P. Plant derived antioxidants-geraniol and camphene protect rat alveolar macrophages against t-bhp induced oxidative stress. Toxic in Vitro 2009; 23(09): 295-301.
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